Infection:Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus.
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Gene expression responses to Zika virus infection in peripheral blood mononuclear cells from pregnant and non‐pregnant women
Article Snippet: ZIKV Puerto Rico strain PRVABC59 (ATCC VR‐1843) was propagated in Vero E6 cells (ATCC CRL‐1586) grown in DMEM medium supplemented with 5% FBS.. Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.
Quantitative RT-PCR:Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus.
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Gene expression responses to Zika virus infection in peripheral blood mononuclear cells from pregnant and non‐pregnant women
Article Snippet: ZIKV Puerto Rico strain PRVABC59 (ATCC VR‐1843) was propagated in Vero E6 cells (ATCC CRL‐1586) grown in DMEM medium supplemented with 5% FBS.. Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.
Control:Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus.
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Gene expression responses to Zika virus infection in peripheral blood mononuclear cells from pregnant and non‐pregnant women
Article Snippet: ZIKV Puerto Rico strain PRVABC59 (ATCC VR‐1843) was propagated in Vero E6 cells (ATCC CRL‐1586) grown in DMEM medium supplemented with 5% FBS.. Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.
Western Blot:Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus.
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Gene expression responses to Zika virus infection in peripheral blood mononuclear cells from pregnant and non‐pregnant women
Article Snippet: ZIKV Puerto Rico strain PRVABC59 (ATCC VR‐1843) was propagated in Vero E6 cells (ATCC CRL‐1586) grown in DMEM medium supplemented with 5% FBS.. Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.
Expressing:Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus.
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Gene expression responses to Zika virus infection in peripheral blood mononuclear cells from pregnant and non‐pregnant women
Article Snippet: ZIKV Puerto Rico strain PRVABC59 (ATCC VR‐1843) was propagated in Vero E6 cells (ATCC CRL‐1586) grown in DMEM medium supplemented with 5% FBS.. Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.
Fluorescence:Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus.
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Activation of PD-1/PD-L1 immune checkpoint by Zika virus
Article Snippet: ZIKV Puerto Rico strain PRVABC59 was obtained from the ATCC (VR-1843).. For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.For virus propagation, C6/36 cells grown for 24 hours were infected with ZIKV and maintained in DMEM with 2% FBS at 28°C in a 5% CO 2 atmosphere.
Article Title: Gene expression responses to Zika virus infection in peripheral blood mononuclear cells from pregnant and non‐pregnant women
Article Snippet: ZIKV Puerto Rico strain PRVABC59 (ATCC VR‐1843) was propagated in Vero E6 cells (ATCC CRL‐1586) grown in DMEM medium supplemented with 5% FBS.. Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.Vero E6 cells at 80% confluency in a T25 flask were washed for 10 min with 1 ml of serum‐free DMEM before infection with 2 ml of ZIKV in DMEM at MOI = 0.5 for 1 h at 37°C.
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